The petition fee in the USCIS fee schedule, any regional center administrative fee, translations, professional fees, and later the removal-of-conditions fee. Keep all of these separate from the at-risk capital.
Separate program fees from the investment, and record which are refundable
The qualifying capital must be at risk in the enterprise, and nothing else counts toward it. Around it sit several distinct costs. The USCIS fee schedule (G-1055) sets the fee for the immigrant investor petition and, later, the fee for Form I-829 to remove conditions, and a regional center project usually charges its own administrative fee that is not part of the invested capital and is often non-refundable.
Certified translations of the title, quota and corporate records, tax and legal advice on the sale, and the immigration advisory work are private costs. Ask each project in writing what happens to the administrative fee and the capital if the petition is denied or the project fails, because the answers differ. A family funding this from a farm sale should also budget for Canadian tax on the disposition before deciding how much capital is actually available.